Study of Conjugation in E.Coli

STUDY OF CONJUGATION IN E.coli AIM : To study the tinct of bacterial conjugation through enthrall of factors,coding for antibiotic resistance. doctrine : From one bacterial punctuate(donor) to another strain(recipient) is a known concomitant that occurs in nature with objective of mixing the cistron pool. In otherwise asexually reproducing organism, DNA steer among bacteria is in different mediating ways: I. Transformation II. Transduction III. Conjugation Conjugation is the approximately widespread routine of transferring genetic material from one bacterial boothular phone to another. It is a process in which unidirectional transfer of DNA is arbitrate by conjugal plasmid DNA or conjugal transposons requiring cell to cell contacts. It was discovered by Lederberg and Tatum. Conjugation is best soundless by considering properties of F-factor, which is a minute circular plasmid DNA, that poop replicate autonomously in the cell or can shuffle into host ch romosome and thus transfer host chromosomal marker. The man-to-man cells with integrated F-factor ar marooned and allowed to form pure colonies. The resulting straincan transfer chromosomal marker at very high frequency and termed as Hfr strain. INTRODUCTION: The E.coli strain A & B referred to as parental strains. give A carries an antibiotic repellent gene in F-plasmid coding for tetracycline, part Strain B is detached of any F-factor, but carries streptomycin skanky gene in its chromosome. consequently both the strains grows on modal(a) containing tetracycline or streptomycin to which they are resistant on conjugating the 2 parental strains, the resulting culture go out be resistant to both the antibiotics, i.e. the conjugate sample when plate on a medium containing tetracycline or streptomycin willing survive suggesting that gene transfer has taken place from Strain A to B. further the parental strain when plated on the uniform medium does not survive as they are sore to either tetracycline or streptom! ycin. MATERIALS REQUIRED:...If you want to experience a plenteous essay, order it on our website: OrderCustomPaper.com

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